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It was calculated for each single replicate.
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In the symetrical experiment, with 104 amoebae, plaques were observed for a single replicate (Figure 1 (D)).
This implies that f for a single replicate must be (1 - w1)/ w1.
For 30 PCR cycles, the ltLR exceeds the mixLR for a single replicate but dips slightly below it at six replicates.
For very low DNA template (15 pg) the ltLR is just under 6 bans for a single replicate, about 6 bans below the IMP.
These two criteria are met by generalizing the f for a single replicate to f = 1/∑ i [ w i /(1 - w i )].
The PLSR and RR-BLUP methods, took less than 1 min to calculate the marker effects for a single replicate of the training data.
Results were obtained by calculating SNP effects for a single replicate of the training data; FR-LS: fixed regression-least squares; RR-BLUP: random regression-BLUP; Bayes-R: Bayesian regression; SVR: support vector regression; PLSR: partial least squares regression.
The authors show that GFOLD outperforms other commonly used methods when used for single replicate datasets.
The maximum running time for single replicate of the GAW15 data set was 3.6 hours with τ = 2 and θ = 50 excluding permutations.
Recently, Feng et al. introduced GFOLD [ 10], a non-parametric empirical Bayesian-based approach, and showed that it outperforms methods designed to work with replicates when used for single replicate datasets.
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