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Inter-assay CVs were also determined for each sample using the total assay activity and, separately, the total cystatin C activity calculated for each separate experiment using the same guidelines described above.
A 10 µM (maximal) isoprenaline concentration was included in each plate for each separate experiment for CRE-SPAP production and 3H-cAMP accumulation, to allow agonist responses to be expressed as a percentage of the isoprenaline maximum for each experiment.
Thresholds for statistical significance for each separate experiment are indicated in Fig. 1A.
Hence, researchers need to generate a new set of control samples for each separate experiment.
A maximal forskolin concentration (3 µM) was included in each CRE-gene transcription plate for each separate experiment.
Nonlinear regression of the concentration response curves was performed with GraphPad Prism (GraphPad) to calculate the EC50 for each separate experiment (9, 11).
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See individual Figures for actual dosage tested in each separate experiment.
RNA from the equivalent of 100,000 unc-4 GFP unc-4 GFPs pooled for each separate microarray experimeneurons
For a separate experiment, the flexor tendons in the right forepaw of each animal were transected and repaired.
Observing some embryonic lethality at 15°C, we tested the effects of pk924 growth at 12°C as shown in Table 2 (see also Fig. 3A for a separate experiment).
Within each of these sites, four plots ranging in size between 8 and 12 m were demarcated for a separate experiment.
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