Participants tested the samples by using the laboratory's routine assays for HEV RNA, in 4 separate assay runs, using fresh vials of each sample for each run.
Each primer pair was used for a separate assay using RNA from CO2 and from O2 samples.
In such instances, TSP can provide an advantage for endpoint SNP genotyping, since the ability of the biphasic mechanism to perform nested PCR in a single-step assay eliminates the requirement for a separate assay to preamplify the target sequence harboring the SNP.
In additional, the MEP score was found to be predictive across 3 separate assay platforms, arguing for its broad applicability and against experimental artifact.
Compared with the reference set of SEA, our DRT reference set specifies the three subtypes of β adrenergic receptor, hence not requiring a separate assay for each one.
Precision was determined by calculation of inter assay CV with three samples of synovial fluid assayed for CRP in 5 separate assays.
Notably, the Sierra Leonean genomes differ from PCR probes for four separate assays used for EBOV and pan-filovirus diagnostics (table S3).
Figure 4B demonstrates the data for T-tau over three separate assays.
The basic principle of the method is to use specific primers and probes for separate assays of the region ('hinge region') of the E2 ORF, most often deleted during integration, and part of the E6 ORF.
Use of the same target did require a separate assay for detection of inhibition to avoid primer competition for target.
In this study, nine independent hybridizations, including one labelled by dye reversal, using RNA samples isolated from four separate assays were performed for each incubation time point.
