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For each section, a minimum of 1000 cells were scored across randomly selected areas of DCIS at a magnification of × 400 using a grid graticule and cell counter.
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A minimum of 1000 cells were counted for each section, and a minimum of 2 3 tissue sections per animal were analyzed.
A minimum of 1,000 cells were counted for each section, and a minimum of two to three tissue sections per animal were analyzed.
For each tissue section analysed, a minimum of 300 muscle fibers were counted.
For each sample a minimum of 5 sections were stained and analyzed.
For each experiment, a minimum of 3 sections from 3 animals per group were used for analysis.
For all antibodies and probes used in paraffin sections, a minimum of 4 cochleas per genotype were prepared for histological analysis.
This was repeated five times for each section (minimum of two sections per donor).
Proteins for 2D-DIGE analysis were isolated from 4 mm of a 14 μm thin cryoconserved section with a minimum of 80% of prostatic glands.
Tissue sections were treated sequentially with PBS containing 0.5%NP-40NP-40and3%H2O2each for a minimum of 15 min and blocked with 2.5% horse serum.
In each cord a minimum of 12 sections was used for each marker.
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