Sentence examples for for each second round from inspiring English sources

For each second round primer pair, optimization of the conditions for PCR and the subsequent HRM step were carried out, noting that the presence of the LCgreen Plus dye increased the primer Tm and thus raised the optimum annealing temperature of the PCR reaction.

Therefore, two second-round PCRs were performed for each first round PCR sample, one with primer mix A and the other with mix B in a volume of 20 μl with nested PCR primers.

Tickets for the first round of the playoffs cost roughly 25 percent more than regular-season games, so the team would generate about $2 million in ticket revenue for each first-round home game.

The same data and methodology that fuels the perfect bracket is used to project a margin of victory for each first-round game, giving us a benchmark to compare the point spread to.

Follow along for analysis on how each first-round pick fits with his new team.

May I make arrangements for additional rooms for second round interviews or greeters?

The top nine in each group qualified for a second round robin, in which each team plays the nine from the other section, with the scores being added to those obtained in the first round robin.

The resulting PCR mixtures were diluted 1 10 with water and 1 μl of each was used for the second round of PCR.

The resulting PCR mixtures were next diluted at a 1 10 ratio with water, and 1 μl of each was used for the second round of PCR.

For each sample three second round PCRs were performed and every reaction was purified individually using QIAquick PCR purification Kit (QIAGEN #28106) and eluted in 50 μl elution buffer (EB).

For 3'-RACE, seven specific primers FLG2-F1∼7 (Table 1) were designed against the nucleotide sequence of the putative exons 2 and 3 of the FLG2 gene and used as sense primers, among which each FLG2-F2∼7 was used for the second round PCR whereas FLG2-F1 was used for the first round PCR.