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Forty-eight hours after transfection, NADP/ NADPH levels for each sample were quantified using the NADP/NADPH assay kit (Abcam) and NAD/NADH levels using the NAD/NADH assay kit (BioVision, San Francisco, CA, USA), according to the two manufacturers' protocols.
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Initially, the total RNA for each sample was quantified using a DU 530 UV/VIS spectrophotometer (Beckman Coulter, Brea, CA).
The total protein content for each sample was quantified using a Bio-Rad kit (Bio-Rad, Hercules, CA, USA).
The samples were shaken for 60 min, and then the total amount of Hp91 in each sample was quantified using HPLC.
Protein in each sample was quantified using the BCA method.
Band intensities for Western blot protein samples were quantified using Kodak Digital Science 1D, version 2.0.3, after imaging with Kodak Digital Science Electrophoresis Documentation and Analysis System 120.
In order to achieve optimal cluster densities for sequencing on the Illumina GAII platform, samples were quantified using two methods.
The normalised samples were quantified using Qubit and three from each genome were selected for library construction.
The DNA samples were quantified using the NanoDrop Reader ND8000 and then standardized to 100 ng/μl as required for the preparation of genomic library for GBS.
RNA samples were quantified using spectrophotometry.
RNA samples were quantified using Multiskan Spectrum (Thermo, Electron Corporation) prior to use.
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