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Primer sequences are listed in Supplementary Table 5. Ct values for each sample were normalized to the average Ct values of four different housekeeping genes (HPRT1, ACTB, SDHA, and GAPDH), and resulting values were used to calculate fold-change of BRCA1 expression for each sample.
Each expression assay was run in technical duplicates and the average values for each sample were normalized to a geometric mean of mRNA levels of reference genes beta 2 microglobulin (B2M, assay Hs00187842_m1, Applied Biosystems) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH, assay 4333764F, Applied Biosystems) run in separate reactions from the same cDNA preparations.
MiRNA levels for each sample were normalized to small nucleolar (sno) RNU43 levels.
Data for each sample were normalized to the respective PROTEIN PHOSPHATASE 2A (PP2A) expression level.
Reactogenic LPS units for each sample were normalized to the OD600 of the culture.
The data for each sample were normalized to an internal standard (GAPDH).
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Cell lysates (10 μg) from each sample were normalized to equal volume and measured in triplicate for NAG activity following the protocol provided by the supplier.
For analysis, the Ct of each sample was normalized to the Ct of the input sample.
sGAG content from each sample was normalized to dsDNA content.
Read counts in each sample were normalized to adjust for sample variations.
The amount of MAPK activity determined for each sample was normalized to total ERK in each sample.
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