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MYCC and MYCN copy numbers were measured relative to the endogenous control RPLP0 and results for each sample were normalised to the copy number of diploid human DNA in Pfaffl equation [ 57].
Ct values obtained for each sample were normalised to those for the house-keeping gene GAPDH; ΔΔCt=ΔCt treatment group) – ΔCt(control group) was calculated and a fold-change in gene expression of the treatment group versus the control group was expressed as 2 ΔΔCt.
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Read counts in each sample were normalised to adjust for sample variation.
The GSH concentration for each sample was normalised to total protein content.
The Cq values for each sample was normalised to the internal control gene Ywhaz (primers provided by Primerdesign) to give the ΔCq value.
The amount of mRNA for LOXL1 in each sample was normalised to the amount of mRNA of the beta 2-tubulin reference gene in the same sample.
Cyclooxygenase-2 mRNA levels for each sample were normalised against GUSB mRNA levels and relative expressions were calculated by using Ct values.
Fluorescence geometric means were used for the analyses and samples were normalised to siNS-transfected control cells, essentially as described earlier.
32 For calculation of relative expression, all samples were normalised to expression of the household gene Abl.
Samples were normalised to 40 ng of cDNA per well and loaded in triplicate for each gene.
To enable comparison between samples, DHODH activity and endogenous orotic acid concentration in HeLa cell and fibroblast samples were normalised to cell number (1.0 × 105).
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CEO of Professional Science Editing for Scientists @ prosciediting.com