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The concentration and quality of RNA for each sample were determined by UV spectrometry (Agilent Technologies, Santa Clara, CA, US).
The particle size distributions for each sample were determined by software Nano Measurer 1.2.5 using enlarged SEM images as shown in Figure 1b,f,f.
The weight average (Mw) and number average (Mn) molecular weights for each sample were determined by gel permeation chromatography (GPC) as described previously (Pinto et al. 2016).
Threshold cycles (Ct) for each sample were determined by Bio-rad iQ5 optical system software Bio-Rad Laboratoriess). Bio-Rad Laboratories
Crossing points for each sample were determined by the second derivative maximum method and relative quantification was performed using the comparative ΔΔCt method according to the manufacturer's protocol.
Relative protein levels for each sample were determined by interpolation of each dilution curve from the standard curve (supercurve) of the slide (antibody) as previously described [ 12].
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The fluorescence intensity of each sample is determined by the integral value for 5 s.
Protein concentration of each sample was determined by BCA Protein Assay Reagent (Pierce).
The RNA concentration for each sample was determined by UV spectrophotometry and quality was assessed by the ratio of 260/280.
The genotype for each sample was determined by amplitude of amplification.
The serum HGF levels for each serum sample were determined by sandwich ELISA.
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