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The raw mapped reads for each sample were counted using htseq-count and read counts per million (CPM) were calculated using the library size normalization function calcNormFactors in the edgeR package [ 32] (http://www.bioconductor.org/packages/2.13/bioc/html/edgeR.html).
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This sample was counted using the 409- to 613-keV energy window with corrections for background, deadtime, and decay.
6) Counting: plasma samples were counted using appropriate standards and blanks for background in a well counter.
The samples were counted using a scintillation counter (PerkinElmer Inc, MA, USA).
For all samples, 10000 cells were counted using a FACSCalibur instrument (BD Biosciences) and analysed using the provided software.
Finally the amount of radioactive polyamine taken up by cells was counted using a Canberra Packard 1900A Scintillation spectrophotometer with a protocol for C. Samples were counted for 10 min or 10000 counts, with a 2% σ error.
50000 cells per sample were counted.
Samples were counted three times, each for 5 min. Counts were corrected for background activity by using blank controls.
Finally, all samples were counted by LSC for 5 h.
Triplicate samples were counted.
Using the alignment, the number of reads that aligned to each gene for each sample separately, were counted (in-house script), and used to produce the counts matrix.
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