Sentence examples for for each sample were cloned using from inspiring English sources

PCR products from three separate PCRs for each sample were cloned using the pGEM-T Easy Vector System kit (Promega, Madison, WI, USA).

Samples which yielded unreadable sequences were cloned using the TOPO TA for Sequencing Cloning Kit (Invitrogen) according to the manufacturer's instructions.

PCR products were cloned, using the TOPO TA cloning kit for sequencing (Invitrogen).

Nested PCR products were cloned using the TOPO® TA cloning kit for sequencing (Invitrogen, pCR®4-TOPO® cloning vector).

Fragments were cloned using InsTAclone PCR Cloning Kit (Fermentas, Lithuania).

The pARF19-3xmVenus-N7ARF19 was cloned using Gateway Multisite cloning technology.

The PCR products were subsequently cloned into the pCR®4-TOPO® vector with the TOPO TA Cloning® Kit for Sequencing (Invitrogen) and multiple clones for each sample were sequenced using the same primers used for PCR.

At least 3 positive clones for each sample were sequenced, and the consensus sequence was used for phylogenetic analysis.

Ten clones for each sample were sequenced in these experiments.

Two clones for each sample were selected.

At least eight clones for each sample were sequenced.