Sentence examples for for each sample were checked using from inspiring English sources

Raw reads data for each sample were checked using FastQC (version 0.10.0, Babraham Bioinformatics, Cambridge, United Kingdom; http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/) to identify features potentially indicative of quality issues (e.g., low-quality scores, overrepresented sequences, and inappropriate GC content).

The quality and quantity of the RNA samples were checked using an Agilent bio-analyzer.

Remaining samples were checked using unsupervised analysis (see Methods).

Integrity of RNA for all samples was checked using the 2100 Bioanalyzer (Agilent Technologies).

RNA quantity and quality of the RNA samples was checked using the Experion RNA HighSense Analysis kit (BioRad, Cat.no. 700-7105), samples with high integrity (RIN > 7) were used for expression profiling.

Contigs for each sample were checked and edited using Sequencher 4.8 (GeneCode, Boston, USA).

The surface compositions of samples, before and after zinc deposition, were checked using Rutherford backscattering spectroscopy.

The log files were checked using TRACER, and the effective sample size for each parameter was found to exceed 200.

The effective sample size for parameter estimates and convergence was checked using Tracer http://beast.bio.ed.ac.uk/Tracer.ac.uk/Tracer

The data quality of log2-transformed RPKM values for these two RNA-Seq datasets was checked using the sample histogram.

RNA integrity was checked using Agilent 2100 Bioanalyzer, and samples with a RIN value of 8 or more were used for amplification.