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Total protein concentrations for each sample were calculated using a BCA protein assay (Pierce), according to the manufacturer's instructions.
Serum total lipids for each sample were calculated using Phillips formula summing triglycerides and total cholesterol.
Serum total lipids for each sample were calculated using the Phillips formula summing triglycerides and total cholesterol.
The copy numbers for each sample were calculated using the C T -based calibrated standard curve method.
The CT) value for each reaction, and the relative level of gene expression for each sample were calculated using the 2−ΔΔ C T method.
Rarefaction curves for each sample were calculated using the aRarefactWin software developed by S. Holland (University of Georgia, Athens; http://strata.uga.edu/software/).uga.edu/software/
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The mean values for the entire sample were calculated using the modal values for each patient.
The SAR values for each water sample was calculated using the following equation (Richards 1954).
The mRNA expression for each gene and sample was calculated using the recommended 2-ΔΔCt method.
This sample was calculated using a formula for finite populations.
Pair-wise Fst values between samples were calculated using EIGENSOFT.
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