Sentence examples for for each sample were calculated according from inspiring English sources
Based on a standard curve generated from genomic DNA of known concentrations of 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78 and 0.39 ng/μL and copy numbers per μL of 16667, 8333, 4167, 2083, 1042, 521, 260, 130 respectively, the copy numbers per μL for each sample were calculated according to the LightCycler 480 software manufacturer's instructions.
For each gene, the nanograms of cDNA of each sample were calculated according to the standard curve method and normalized to GAPDH.
The protein concentration of each sample was calculated according to a standard dilution series.
The size of the nuclear genome of each sample was calculated according to standard procedures [45].
The relative ratio for each sample was calculated according to the formula: (conc. wpgrp1 (sample)/conc.
The concentration for each sample was calculated according to the standard curve, after subtraction of the blank values.
The viremia level of each patient sample was calculated according to the standard curve.
The viremia level of each serum sample was calculated according to the standard curve.
Copy numbers of the target microbiota for samples were calculated according to the standard curves.
The crystallinity index (CI) for all the samples were calculated according to the procedures previously described [ 46, 51].
The values for the BDNF concentrations in samples were calculated according the formula generated from the trend line of standard curve.
