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The area associated with dark Iron III deposits was traced and the total area values for each sample were acquired using Neuroexplorer software (MicroBrightField).
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The IRMs for each bulk cube-shaped sample were acquired using a Magnetic Measurements MMPM10 pulse field magnetizer.
FTIR spectra for all other samples were acquired using a Spectrum One FTIR instrument (Perkin Elmer, Waltham, MA, USA).
Samples were acquired using a LSR-II Flow Cytometer System (BD Biosciences).
Samples were acquired using Sony Spectral cell analyzer SP6800 (Sony Biotechnology Inc., San Jose, CA).
Data from labeled samples were acquired using a BDTMLSRII cytometer (BD Biosciences) followed by analysis using the FlowJo software (Tree Star).
Color images of pork loin samples were acquired using a CVS.
Images of immunofluorescently stained samples were acquired using a Fluoview confocal microscope (Olympus, Melville, NY).
All samples were acquired using identical voltage settings.
For each sample, ten thousand events were acquired using the CellQuest software (BD) and data analysis was performed using WinMDI 2.9 software.
The area of positivity was determined using an isotype matched control Ab. 104 events for each sample were acquired.
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