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The total number of events to be measured for each sample was set to 10,000.
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The number of random sampling was set to 10000.
The FCs for control samples were set to zero.
Sampling frequency was set to 0.033 fps for fluorescent imaging.
The instrument setting for FCM was set to analyze 20,000 cells per sample.
Mean corpus callosum thickness across all control samples for the analysed anterior posterior interval was set to 100%.
The overall sampling fraction of species was set to 0.14.
One sample was set as the calibrator for the analysis.
For samples with pre-growth overheating, the sample temperature is set to T=670 °C for 120 s.
Live cell counts for each sgRNA sample were normalized to live cell counts obtained for sgAAVS1 control transduced samples, which were set to 1.
Here the peak Si absorption of green pixel of Sample A is set to be 1.0 for normalization.
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