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The conventional method applies interpolation for each sample separately.
Patient/tumor characteristics are presented in Table 1 for the whole series and in Table S1 for each sample separately.
First, we compared GLAM predictions and StaRT-PCR quantifications for each sample separately, to assess the performance of the model at the sample level.
UTAX and RDP were executed for each sample separately.
Peaks of methylation were identified for each sample separately.
Reads were mapped for each sample separately to the assembled transcriptome.
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These RNAs were used to prepare biotylinated cRNAs for hybridization, each sample separately hybridized to one array.
For both species, we tested whether the groupings that yielded the most likely genetic structure pattern with regard to microsatellite and sequence-based marker results (regarding models As1 and As6 for A. schaueriana and model Ag1 and considering each sample separately for A. germinans) presented signs of recent demographic change.
Considering both the genetic structure identified herein (in model As6 and when considering each sample separately for A. germinans) and the a priori scenarios, namely models Ag1 and As1, based on previous studies [ 17, 18, 20, 25, 26, 40], we observed different indications of recent demographic expansions in both species from both the a priori and a posteriori hypotheses.
Rseq was used for RPKM based expression measurement on each sample separately.
To confirm the one- and the two-factor models we conducted Confirmatory Factor Analyses (CFA) with follow up visit data using structural equation modeling for each country sample separately and across all countries pooled (see Additional file 2 for the Path diagram of the CFA).
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