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To identify entry points of pathways, for each reactions of the macrophage model under gene control flux variability analysis was performed to determine in which direction the reaction can carry a flux.
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Modified Arrhenius equation applied for each reaction.
The energy barrier for each reaction is determined.
The advantages and disadvantages are discussed for each reaction.
Three replicates were carried out for each reaction.
The method is tested for each reaction as follows.
Apparent activation energies were also determined for each reaction.
For each reaction, four reaction pathways were examined structurally and energetically.
The transition state for each reaction was determined, and the intrinsic reaction coordinate was analyzed.
For purified protein samples, RNaseI (Thermo) and RNaseA (Thermo), 50 ng was used for each reaction.
The inlet feed was fixed for each reaction as shown in Table 1.
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