Sentence examples for for each primer set from inspiring English sources

The number of cycles and the annealing temperature was optimized for each primer set (Table 2).

In each run, negative controls (no cDNA) for each primer set were included.

A standard curve was included for each primer set.

Briefly, the E for each primer set was recorded per sample and an average E (EA) was then calculated for each primer set.

Triplicate reactions from two independent experiments were run for each primer set across all templates.

Annealing temperatures and cycling conditions were determined empirically for each primer set.

A standard curve was plotted for each primer set, as described elsewhere [20].

At least two independent replicates were performed for each primer set.

PCR cycles and amounts of templates were optimized for each primer set in pilot experiments.

RT-qPCR of pooled cDNA was used to generate a PCR product for each primer set.

Standard curves were obtained for each primer set with serial dilutions of plasmids containing the amplification product.