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The time may vary for each oven or for each pan.
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Screening of the library was performed as described elsewhere [ 35, 36], except that 10, 15, and 30 washes were performed for each successive round of panning.
For each panning step, 50 µl of CsA-immobilized resin slurry was added to 1 ml of the T7 phage (>1011 pfu) followed by incubation for 8 hours at 4°C.
Bake each pan for approximately 45-50 minutes.
For each screw pan and press-fit cup, five measurements were made on specimens with normal bone density and five on osteoporotic specimens.
To reduce the amount of antigens needed for each bio-panning, all antigens were immobilized on wells of 96-well ELSIA plate (except for cancer cells that were grown on 5-ml flask).
The pilot study should develop guidelines and best practice for Ethics for pan European studies.
For each sample aluminum pans suitable for TGA analyzers were filled with aprox.
The input phage number (1×1011 pfu) was kept constant for each round of panning.
The input titer of phage used for each round of panning was determined each day, and always comprised between 10 and 10 phage particles (data not shown).
For each round of panning, 150 μL of the amplified phage library (about 10 PFU) and 10 μL of methanol were first added to the 2 BDE C1 BSA-coated wells.
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