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Three sections were measured for each mouse.
(C) Urine-derived cell numbers for individual mice counted three times for each mouse.
Melting curve profiles completed on each amplified sample revealed the genotype for each mouse.
For each mouse, 3 4 bregma-matched sections comprising the dentate gyrus or the frontal cortex were imaged.
For each transition between consecutive activation states, we further calculated the transition rate, for each mouse individual (see panel b).
For each mouse, cell abundance was collected (either using CPM or flow cytometry) along the various cell activation states.
The sera (300 μL for each mouse) were collected under anesthesia by extracting the eyeball blood.
These parameters were averaged for each day and for each mouse.
Date and cause of death were recorded for each mouse.
The starting arm for each mouse was randomized.
We also calculated gender-reference values for each mouse strain.
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