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Tg 189N3 110±44 versus 100±7; p<0.26 by Student's t test n = 5 for each) and Ts65Dn (111±7 versus 100±3; p<0.17 by Student's t test n = 5 for each) mice have also the same CBS activity than that of non-transgenic mice.
Two slides were prepared for each mice; each slid content two sections.
MIA PaCa2, CFPAC-1 and PaCa3 cells (5 × 10 cells for each mice) were s.c. injected into female nude mice (4 weeks of age, Harlan, Italy).
The infected K19-Wnt1 mice were examined at 8 and 20 weeks after the H. felis inoculation (n=3 for each mice).
The ROI analyzed for the ΔR/R measurement, were selected based on the glomerular map obtained for each mice at the lowest dilution of four odorants used.
(B) Size (diameter in μm of each metastasis) and number of metastasis (specified for each mice) was evaluated using the Olympus DP-Soft morphometry program in an OlympusDX51 microscope.
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4 mice were used for each group and 20 40 myofiber diameters were measured for each mouse.
These parameters were averaged for each day and for each mouse.
Melting curve profiles completed on each amplified sample revealed the genotype for each mouse.
The sera (300 μL for each mouse) were collected under anesthesia by extracting the eyeball blood.
Five pairs of mice were used for each time point, and more than 10 sections for each mouse were analyzed.
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