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For each mate choice trial a large and small male were randomly selected and placed in a plastic container with clear mesh sides.
For each mate trial between a single male and a single female, males were allowed three copulatory attempts.
Sexual selection estimates (W) are given for each mate type, in males and females separately, relative to the type with the highest sexual fitness [ 4].
For each mate choice design we used the program JMating to calculate the index of sexual isolation I PSI [ 57, 58].
For each target superscaffold group, a score is calculated by summing the value for each mate pair in that group (see below for details of how the value is determined).
For each mate pair library, we estimate the mean insert size gmean and the insert size range [gmin, gmax] and using these values we compute minimum and maximum suggested distances between the contigs based on each mate pair.
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In detail, the link-score combined PEMPs that had unique, high-scoring alignments for both ends of each mate pair sequence, but with the two end sequences aligning to different non-adjoining superscaffolds.
Typically each male has zero or multiple matings to make, and there is a ranking for each mating, rather than for each male, such that matings for a given male are generally dispersed throughout the ranked list.
For each mate-pair, the algorithm scores all possible ungapped alignments (this includes alignments shorter than the read length, which are produced when a fragment is fully sequenced resulting in and additional synthesis reactions proceed past its 5-prime end, base-pairing with the Illumina adapters).
The number of children for each mating was drawn from a Poisson distribution with mean 2, and children from all marriages comprised the second generation.
The number of sperm in the spermatophore was counted for each mated snail.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com