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De novo transcriptome assembly was carried out for each mapping file separately using cufflinks (v1.3.0).
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JF and SF models for multifamily and biparental mapping populations were fit using each thinned map separately, and phenotype values for the validation set lines were predicted separately for each reduced map (File S11 and File S12).
The schema for this CV mapping file is shown in Fig. 2.
For each row, the same mapping file was used, but a different annotation was implemented in the program Cufflinks in order to generate FPKM values for each gene.
Corresponding UniProt Accession Name (Uniprot_AC) was obtained for each mapped Entrez Gene ID between the species using the ID mapping file for each organism from 1.
For each species, map-files of the individual geographic extent of its distribution were available, including information about the presence, origin and season.
A community matrix was then created from the mapping file for each locus by retrieving the sample information in each sequence label and cross referencing with OTU identity of the read.
The authors thank Dr. Mark Lohse of Max Planck Institute (Germany) for generating a draft PGSC mapping file for MapMan analysis in potato.
Sequence reads of 100 bp, either obtained by simulation (see below) or by Illumina sequencing of DNA from sausage meat (see below), were initially mapped against reference genomes using the algorithms BWA (V 0.7.0; [ 29]) or CUSHAW [ 30] resulting in a SAM file for each mapping.
Authors acknowledge the support from MapMan team for annotation and generating the mapping file.
BAM files for each mapping were then exported and input into the samtools package (Li et al. 2009) for local phasing, variant calling, filtering, and consensus calling.
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