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After selection, an aliquot of the CD133+ cell fraction was analyzed to assess purity, which was determined for each isolation experiment.
For each isolation 50mg (dry weight) seeds were used.
The quantification cycle (Cq) was then averaged for each isolation method for each species.
For each isolation, four confluent ∅ 15 cm dishes with confluent BAEC were treated as indicated.
The seeding density for each isolation method was as follows: CM-714 cells/cm and ED-2857 cells/cm.
For each isolation, 200 μl of serum was used resulting in 50 μl of nucleic acid extract.
Similar(48)
There was no change in protein profiles or plasmid DNA banding patterns during this investigation compared with those observed for each isolate following original isolation from patients described previously [ 14, 15].
For ChIP experiments, after each isolation cells were equally divided for control and heat shocked at 43°C group.
All four isolation beds shared a common inlet port with different outlet ports for each cell isolation unit.
The design of the fault isolation decision scheme is based on the derivation of appropriate adaptive thresholds for each fault isolation estimator.
For each DNA isolation, three 9-mm punches (1.9 cm) were sampled from each plant.
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