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One spacer is inserted for each insertion event.
For each insertion sequence, the genomic position of the insertion sequence and of the inserted position in the cognate genome are given.
For each insertion branch in the comprehensive tree into which the candidate subtree shall be inserted, we once again conduct a case analysis to determine if the specific SPR move on the comprehensive tree also induces a change in the gene trees.
Biotin‐labeled run‐off in vitro RNA transcripts, representing the neighboring chromosomal DNA for each insertion remaining in the population, are generated using T7 promoters located at the ends of the transposon.
The breakpoints and their environment were unique for each insertion.
Ideal cloning strategies contain only unique restriction sites (only present once) to ensure that cloning is directional and straightforward, hence requiring two unique sites for each insertion event.
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Applying a checklist for each CVC insertion, bearing in mind a) the use of maximal sterile barriers during insertion, b) appropriate hand hygiene, c) insertion site disinfection and antisepsis, d) choice of catheter insertion site and reassessing the need for the central access on a daily basis.
The specificity constant (kcat/ Km) provides a measure of the catalytic efficiency for each dNTP insertion reaction and allows comparisons to be made between dNTP insertion events.
This yielded 11 transcript isoforms for each Ds1 insertion.
Moreover, due to the amount of information provided for each nuclear insertion, this database should play an important role in designing evolutionary, phylogenetic and epidemiological studies.
We randomly chose to duplicate 1 10 bases for each simulated insertion.
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