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The protein input for each immobilization method was varied between 0.001 and 0.133 gprotein/gparticle.
For each immobilization, a theoretical maximal response (Rmax) was calculated for all compounds according to the formula: Rmax = (MWcompound/MWprotein) × RI, where MW = molecular weight and RI = immobilization level.
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For each type of immobilization, the experiment was repeated at least three times.
Estrous cycles were monitored for each rat with immobilization timed to coincide with the onset of estrus, leaving most rats in proestrus 4 days after the end of stress.
To optimize the amounts of proteins used for immobilization, each recombinant protein at 0.25, 0.5, 1.0, and 2.0 μg/ml was analyzed.
The reaction has been employed for glycoproteomics studies xiv) and for immobilization of azide-labeled proteins on surfaces.
Each patient was screened for spinal immobilization using an institutional spinal immobilization guideline in place since 2002.
One primer in each pair was biotinylated for later immobilization.
T-GCH1 is immobilized at a surface density of ∼1500 RU via His-capture, and GFRP and L-phe are introduced in varying concentrations (400 25 nM) (n = 4 for each experiment at varying immobilization patterns and surface densities).
Primers are indicated in Table S1 from Additional File 1 and one primer in each pair was biotinylated for immobilization.
The incorporation of functional groups for immobilization or for linkage to fluorophores, nanoparticles, radiolabels, etc., is well controlled.
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