Your English writing platform
Discover LudwigExact(7)
The average observed linear attenuation coefficient (LAC) values with a unit of cm−1 (here multiplied by 1,000) are indicated for each flask.
Dry weight was determined by filtering the biomass through normal filter paper and the cell clumps were removed from the immobilization materials to obtain the fresh weights of biomass for each flask.
For each flask, a 0.2-μM Millipore filter was used on the inlet line and the outlet line was loosely plugged with cotton.
For each flask, 10 μl of Oligofectamine reagent was incubated with 40 μl of serum-free medium for 10 min at room temperature.
One milliliter of β-estradiol/diosgenin (a final concentration of 0.2 mg/ml) for each flask was added for biotransformation analysis after E. coli IBL161 was induced by IPTG at 25°C for 2 h.
The number of stably transduced cells, the number of flasks reseeded and the number of colonies obtained for each flask at 38°C are shown in Additional File 2. The flasks which contained more densely growing/bigger colonies (indicated in red in Additional File 2) were trypsinised, replated and used for extracting genomic DNA when confluent.
Similar(53)
Add 3 mL of media for cells obtained from each flask.
After 2, 6 and 24 h, an aliquot of 10 mL was taken from each flask, centrifuged for 5 min at 10 000 r.p.m. and then analysed for NO3− as described above.
Subsequently, 1/10 of the cells of each flask were seeded into another 25 cm flask and incubated for further 72 h.
After cells from each flask had been reached the confluence, they were harvested and replated in two new flasks for expansion.
For fungal inoculum propagation, each flask from the above step was transferred to 10 L capacity Erlenmeyer flask contained 1 kg sterilized moistened SBP, and then incubated statically at 32 °C for 4 days.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com