Among mothers with children 6-23 months, a score of 1 was assigned for each food group fed, for feeding the recommended frequency or more and for breastfeeding.
For each blood-fed sample, total RNA was extracted from batches of approximately 10 15 females.
4 For each SW-fed wetland, we assumed that water consumption upstream would reduce the inflow into the wetland and thus cause biodiversity damage.
In phenobarbital and high cholesterol feeding experiments, samples from 8 mice were pooled by two into 4 pools for each control, cholesterol fed and phenobarbital treated group.
This occurred in fasted groups only for ADRB2 and for fed and fasted groups for GRM8.
One microgram of double stranded DNA for each original library (sugar-fed, blood-fed, L. major-infected) was fractionated using a Chromaspin 1000 column (Clontech Laboratories, Mountain View, CA) into small (S), medium (M) and large (L) transcripts based upon their electrophoresis profile on a 1.1% agarose gel.
Replicate analysis of a stream sediment standard gave precisions of 3% for FeA and 10% for FeD using this sequential extraction.
The specific growth rate for each strain in the fed-batch phase was set below μmax/2 to avoid overflow metabolism [ 30].
For each condition (fasted, re-fed), non-redundant reads from all five biological replicates were pooled into a single read set, and initial peak-calling was performed with HOMER v3.0 (5% FDR) [ 24], using a large pool of previous experimental inputs as the background control.
