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Distance migrated was calculated by subtracting the average distance between wound edges from that at time 0. For each experiment a total of 12 wounds were measured per group, and each experiment was repeated three times.
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We included three replicates for each dose for each experiment, conducting a total of three experiments (nine replicates total for each dose) for this assay.
For 2D experiments a total of 50,000 HDFs were seeded per well of 12 well plates.
For co-immunoprecipitation experiments, a total of 1.8 mg of protein from HT22 cells was used.
There were three independent experiments for each compound, for a total of 36 plants.
For each condition, a total of 35 50 cells were scored in 5 independent experiments.
For the second round of experiments, a total of 6 mock IPs were performed.
The eluted peptides from each IMAC column were analyzed by one MudPIT experiment for a total of six MudPIT experiments.
Each subject performed ten trials of two experiments for a total of 80 experiments.
For each probe, 3 9 independent experiments with 2 10 cells per experiment and a total of 21 43 cells were performed.
Each contrast was tested for 2 trials, and the experiment involved a total of 18 trials.
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