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After designated time, the flasks contents were filtered, centrifuged (8000×g, 10 min) and residual dye concentration was monitored at respective wavelengths (HALO DB-20).The decolorization efficiency of MnP-I-PVAA and F-MnP for each dye was calculated using relation in Eq. (2).
The decolorization efficiency of free and immobilized MnP for each dye was calculated using the relation given in equation 2.
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The percentage of desorption for the dyes was calculated using the following equation [61]: {text{Desorption}}left( % right) = frac{text{Amount of dye desorbed}}{text{Amount of dye adsorbed}} times 100.
The amounts of the two dyes were calculated using Eq. (6).
The percentage dye uptake was calculated using Eq. 1.
The dye concentration was calculated using ɛ=5700 M−1 cm−1 at 340 nm.
Dye velocity was calculated using this time delay and the measured distance between the two ROIs.
The dye uptake capacity for theadsorbate was calculated using a simple concentration method.
The effective diffusion coefficient, Di was calculated using the Boyd constant and was found to be 5.05×10−04cm2/s for an initial dye concentration of 50 mg/L.
A background offset and a scaling factor for each array and dye channel were calculated using the least squares regression procedure, then the generalized log-transformation was applied.
The dissociation constant (KD) value for the complexation between β-CD and AY99 dye can be calculated using the Benesi Hildebrand equation (Velusamy et al. 1996).
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