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The distribution of permanence time for the total thymocytes and for each differentiation stage (DN, DP, and SP cells) can be seen in Figure 4.
Only GRG with a change in mRNA expression of at least two folds was retained for each differentiation pathway.
However, as there were only two biological replicates used by Glover et al. [ 19] for each differentiation protocol these results may be questionable.
We therefore examined several cell-type-specific markers for each differentiation stage: the hlh-8 gfp hlh-8 gfpecifically expressed in undifferenthated cells of M liseage (Harfe et al., 1998b) and an egl-15::gfp reporter which ispecificallyy expressed in vm1 cells in egg-laying system (Harfe et al., 1998a; Hundifferentiated2004).
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Would the days of maturation be very different due to the differentiation method; therefore, such time-course should be performed for each new differentiation protocol?
(C ) Quantification of flow cytometry for each day of differentiation in both Gsc- and (D ) Gsc+ differentiating cells.
The percentages of methylation at each CpG site in the Ins2 promoter are shown for each stage of differentiation.
For each SNP, a differentiation index (DI) was calculated between the southern and the northern pool as follows.
For each species, population differentiation (FST) averaged across all loci, and pairwise FST estimates between all population pairs [ 79] were calculated using fstat 2.9.3.2.
For each dataset, genetic differentiation (F ST ) between the clades identified through TCS and phylogenetic analyses were estimated by also using DnaSP.
For the differentiation assay, each of the Thermanox™ coverslips coated with a graphene film was placed into an individual well of a 12-well TCPS culture plate.
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