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Participants earn $6 for each correct sequence and $2 for each incorrect sequence.
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Sequences were then determined via multiple clones produced from each PCR product, a standard aDNA protocol for insuring correct sequence determination [ 12, 22] (additional fig. 1, 2, 3).
Agreement was similar for erosion size (78%) and better than for the correct sequence (58%).
The positive clones were checked for the correct sequence and orientation by restriction enzyme digestion and by sequencing.
The FixPred pipeline attempts to correct erroneous sequences in several steps, starting with the simplest solutions (finding experimental evidence for the correct sequence version in existing protein or cDNA and EST databases), progressing to more time-consuming gene-predictions.
The construct was tested for correct sequence, linearized with BlpI, and used for transforming S. pombe strains by homologous recombination.
aR all purified and checked for correct sequence plasmids were used as template for transcription reaction by TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific, USA) as instructed by the manufacturer.
Further, all spectra corresponding to phosphopeptides were manually inspected for correct sequence identification validation and site assignment based on the presence of site determining fragment ions.
Peptides were characterized for purity and correct sequence by MALDI-TOF LC-MS and Edman degradation.
Sequence data was analysed using 4Peaks (version 1.7.2) software and entered into NCBI C.elegans BLAST for confirmation of correct sequence.
Full credit (a score of 1) was given for each correct, 5-key press sequence; partial credit was given for sequences with four consecutive key presses (score of 0.8) and three consecutive key presses (score of 0.6).
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CEO of Professional Science Editing for Scientists @ prosciediting.com