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Uptake and depuration of nanoparticles in test organisms have to be determined for each commercialized functionalization of the nanoparticles as differently functionalized nanoparticles can exhibit different bioavailability to, e.g., D. magna even though the core material is the same.
We further suggest that the importance of considering the impact of shading in algal tests is made more detailed in the guidance and that it is specified that determination of uptake, depuration and trophic transfer of nanomaterials for each commercialized functionalization of the nanomaterials is required.
Determination of uptake and depuration of nanoparticles in test organisms, furthermore, has to be taken into account for each commercialized functionalization of the nanoparticles and trophic transfer as both exterior bound and/or accumulated nanoparticles in prey organisms will be available for predator organisms, thus potentially increasing the toxicity compared to aqueous exposure.
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CEO of Professional Science Editing for Scientists @ prosciediting.com