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In addition to sequence information, this second generation platform generates quality scores that are analogous to Phred scores (which assign a probability to the four possible nucleotides for each base in the sequence) [ 15].
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All sequencing platform vendors provide quality scores for individual bases in the sequence reads.
For each base pair in the genome, the number of overlapping sequence reads was determined.
CAP3 may yield conflicting bases in the sequence generated for the contig.
Finally, any remaining ambiguous bases in the sequence were targeted for resequencing.
Primers for MaT were based on the sequence published in the NCBI database (AF489108).
For each alignment, the identity level was computed as the fraction of bases in the reference sequence that are paired with identical bases in the target sequence.
Its order is based on the sequence in which the factors impact sports performance.
A 1.5-base allowance was made to compensate for the nucleoside eliminated in the sequencing reaction.
These natural milk protein genetic sequences are not actually from the animals, but based on the sequence found in animals.
Even a small change in the sequence of base pairs can cause havoc.
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