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This small scale transient transfection method can yield on average 10 μg/ml and so could be used in cases where very small amounts of proteins are sufficient for downstream work (e.g. protein micro-arrays) or could be used as a pre-screen prior to larger scale culture.
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All lines produced the expected results, except RSL135, which was heterozygous for all markers and was therefore excluded from downstream work.
This paste was then aliquoted and resuspended in pure ethanol to obtain stock solutions (50 mg/ml) of tertiary crude extracts for downstream experimental work.
The deal, which also moves the company into downstream work, is expected to close next quarter.
As a result, this work illustrates these forms of DNA damage can have major consequences for downstream interpretation as not all actionable mutations could be validated.
This work demonstrates the feasibility of oscillatory flow based platforms for the development of continuous protein crystallisation as for downstream bioseparation.
This matrix was utilized for downstream analysis.
After preprocessing, SINCERA pipeline30 was used for downstream analysis.
We are currently working on expanding our code space to include up to 1,000 distinct spectral codes by using additional lanthanide species, as well as on functionalizing beads for downstream assays.
The river is a major tributary to the Amudarya – the main water vein for downstream Uzbekistan.
The project will also help renew the water supply for downstream communities.
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