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Combining both types of features provides a quick and low-cost way to pinpoint the potentially most interesting variants for downstream validation and functional studies.
For these 20 genes, a total of 175 SNPs were discovered and 72 were included in the bead array for downstream validation.
30 Both studies used the Katz Activities of Daily Living Instrument 31 3 months after HARP assessment for downstream validation purposes.
Such an approach can provide guidelines for the interpretation and prioritization of predicted somatic variation from matched tumor-germline exome-sequencing data for downstream validation studies (Table 6).
The current protocol is not intended to definitely prove the past selection in any given chromosomal region, but rather to only find candidate regions for historic selection genome-wide for downstream validation tests.
The researcher has little choice but to attribute quantitative variation to biological noise and sample variability and only select proteins with the most significant expression differences for downstream validation experiments.
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Tail DNA was also extracted from the parents and used for downstream validations.
For a newly emerged strain, the B-cell epitope mapper workflow performs in silico prediction of its cross-neutralization based on existing nAbs and provides preliminary results for the design of downstream validation experiments.
Predictions with a 'somatic score' of 40 or greater were considered for subsequent downstream validation and analysis steps.
Predictions not labeled as 'REJECT' were accepted as confident somatic mutation predictions, and considered for subsequent downstream validation and analysis steps.
Predictions with a joint probability of having a variant in the tumor sample and no variant in the germline sample (p_AA_AB) of 0.9999 or greater were considered for subsequent downstream validation and analysis steps.
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