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In eukaryotic nuclei, the 3′ ends of mRNAs are generated by cleavage and polyadenylation, and the same sequence elements that specify that process are required for downstream release of the polymerase from the DNA.
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Mean travel times of release groups were calculated for downstream (from release to detection near the river mouth) and coastal (from river mouth to ocean stations at NSOG, QCS or JDF) portions of the migration.
Lake Mead and downstream releases from the dam provide water for both municipal and irrigation uses.
The sum of the presented data indicates that the methods developed in this study allow for spatial annotation of N-glycans on FFPE tissue sections as well as straightforward in-solution N-glycan release for downstream characterization by LC-MS/MS.
Changes in wet season rainfall (80% of the annual total) dominate the total annual amount of water released for downstream flow, but dry season rainfall (20% of the annual total) changes are also very important as they affect onset and the duration of the period when there is no runoff.
To prepare for downstream assays, organoids are then released from Matrigel by incubating at 37 °C for 40 min in 50 µl of 5 mg/mL dispase (Life Technologies 17105-0411).
Hydrolysis of both cellulose and hemicellulose in pretreated biomass via enzymatic action is critical to release monomeric sugars for downstream fermentation.
Therefore, with the response to stimulus, such as pathogen attack, JA activation will be mediated by 26S proteasome degradation of JAZ repressors that consequently releases MYC2, allowing for downstream transcriptional activation of JA.
► The release of maturation factors by AAA-ATPases is critical for downstream maturation of pre-60S particles.
Or are the release events coupled to energy-consuming structural rearrangements, which are necessary for downstream maturation reactions?
Local inflows for downstream reservoirs (viz., Maithon and Panchet) and Durgapur Barrage were computed from information on routed release at concerned reservoirs from upstream reservoir and recorded inflow therein.
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CEO of Professional Science Editing for Scientists @ prosciediting.com