Sentence examples for for downstream assays from inspiring English sources

To prepare for downstream assays, organoids are then released from Matrigel by incubating at 37 °C for 40 min in 50 µl of 5 mg/mL dispase (Life Technologies 17105-0411).

We are currently working on expanding our code space to include up to 1,000 distinct spectral codes by using additional lanthanide species, as well as on functionalizing beads for downstream assays.

The electrophoretic sample separation of nucleic acids will continue to be an important part of the sample-to-answer workflow, providing cleaner analytes for downstream assays like clinical diagnostics, polymerase chain reaction (PCR) and sequencing.

The ideal for characterizing therapeutic sensitivity would allow for: real­time decision making, identification of rare subpopulations with therapeutic resistance, analysis of very small samples (e.g. MRD), and maintains viability individual cells for downstream assays to characterize phenotypic, genotypic, transcriptional and other determinants of sensitivity.

GFP+ cells were sorted after 2 days for downstream assays.

PMNs were suspended in desired medium for downstream assays, and the purity/viability was typically >90% as evaluated by flow cytometry.

The goal of this stage of analysis is to determine if the sequencing was done with the required depth of coverage and to prepare BAM alignment files for downstream assay-specific analyses.

A number of reports have investigated the level of dissection precision required for a variety of downstream assays [ 4, 11- 17].

By virtue of its universal nature in preserving whole blood, our approach is generally applicable to any cell isolation protocols for any downstream assays.

In this setting, our method produces a population of amplified RNA having minimal and consistent amplification bias, orientation compatible with both spotted cDNA and oligonucleotide arrays, and quantity sufficient for several downstream assays from a single amplification.

However, the ideal option would be to extract GPCR from the membrane, for purification or downstream assays, without the requirement for detergent.