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For double knockdown experiments, a cumulative MOI = 50 is used.
For double knockdown experiments, SMARTpool siRNA designed against Twist1 from Thermo Scientific was used.
For double knockdown of TGF-β1 and TGF-β2, 250 μL of each lentivirus-containing medium were used.
Unless otherwise stated, throughout this paper the siRab8 for double knockdown of Rab8A and Rab8B means a mixture of siRab8A and siRab8B.
Out of the two combinations tested for double knockdown, miRNA #2 against TGF-β1 and #2 against TGF-β2 showefficientent silencing in both A549 and HFL-1 cells.
For double knockdown experiments, Bim expression in control shRNA or Puma shRNA-transduced Caco-2 cells was silenced using Bim siRNA (L-004383-02) or control siRNA (D-001820-01) (Dharmacon, siGenome SMARTpool).
Similar(52)
The expression of the TCR on T cell surface of double knockdown cells was similar to each single knockdown population.
For the double knockdown of β-arrestin-1 and -2, 1.5 ml of each virus of specific clones were transduced in 2 ml of HMC-1 or LAD-2 cells (5×106).
Genetic interaction scores are computed for each double knockdown experiment.
Our preliminary experiments have also indicated that the tandem siRNA expression vector designed for the double knockdown of mouse FUT8 and GMD also worked in mouse myeloma cell lines NS0 and SP2/0.
For example, double knockdown of the intermediate compartment and Golgi-tethering factor transport protein particle (TRAPP) together with the ER SNARE protein Sec22 resulted in reduced pathogen replication efficiency.
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