Sentence examples for for double immunolabeling from inspiring English sources

For double immunolabeling, primary antibodies (anti-DNMT3a and anti-NeuN as a neuron-specific marker [53], [54]) were labeled with the Zenon Alexa Fluor 594 Rabbit IgG Labeling Kit or the Enhanced Zenon® (Alexa 488) Mouse IgG Labeling Kit (Invitrogen, Carlsbad, CA) and applied at 1∶500 final dilution to the slides for 4 hrs.

For double immunolabeling, Fas/CD95 and FADD proteins were detected following 1 h incubation each with specific anti-Fas/CD95 (C-20) rabbit antibody (Santa Cruz Biotechnology) and anti-FADD (clone-1) mouse monoclonal antibody (BD Transduction Laboratories, Lexington, KY) (dilution 1∶10 in blocking solution).

For double immunolabeling, both primary antibodies were added simultaneously.

Five hippocampal slices from each animal were systemically and randomly selected for double immunolabeling with antibodies against glial fibrillary acidic protein (GFAP) and Cav1.2 or Cav1.3.

For double immunolabeling, a mixture of donkey anti-rabbit AlexaFluor®488 (dilution 1 250) and donkey anti-mouse AlexaFluor®594 (dilution 1 250) antibodies (Molecular Probes) were applied.

For double immunolabeling studies, fluorescein isothiocyanate-labeled goat anti-rabbit immunoglobulin G, or rhodamine-labeled donkey anti-mouse immunoglobulin G, was incubated as a secondary antibody for 60 min at room temperature in the dark.

Indirect immunofluorescence method was used for double-immunolabeling.

For KiSS-1 and ERα double immunolabeling, slides were incubated for 72 hr at 4°C with a mixture of polyclonal anti-KiSS-1 (as above, 1 1,000) and monoclonal anti-human ERα antibody (Dako UK Ltd ,Ely, UK; 1 200) in PBS containing 2% NGS.

Fig. 1B illustrates double immunolabeling for TH and Sim1 on fixed paraffin sections from mouse midbrain at the area of substantia nigra pars compacta (SNc) and ventral tegmental area (VTA).

For LHβ and ERα double immunolabeling, we sequentially immunostained sections using nickel-intensified diaminobenzidine (NiDAB) and diaminobenzidine (DAB).

To further confirm the neuronal distribution of p75NTR in HD human brain double immunolabeling for p75NTR and DARPP32 was performed in HD caudate-putamen brain sections.