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For DND MEC-contaminated sites, the determination of 'clean' is made by the Explosive Risk Rating Sub-Committee (XRRSC) on a site-by-site basis.
"That decision has not yet been made," said Captain Alexandre Munoz, a spokesperson for DND.
"The costs of the support provided was re-funded to DND under a Provision of Service Agreement, which is the standard agreement that is used whenever DND agrees to provide a service to a non-Defense or non-Government of Canada entity," said a spokesperson for DND.
Finally, the 'tBlastn for Dnd' page, utilizes a NCBI tBlastn-based tool that we developed to predict potential Dnd proteins in user-supplied nucleotide sequences.
Strain that did not yield any of the expected amplicons was considered as negative for Dnd genotypes.
On the other hand, if the dnd-encoding GI is absent, a short bridge amplicon spanning the GI insertion site will be generated for Dnd− M. abscessus strains [ 12].
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The sputum of CF patients is extremely rich in DNA, which may constitute an important source of nutrients for dnd-positive strains.
A standardized naming system for dnd-encoding GIs was also proposed in this study to avoid confusion.
Despite the highly conserved upstream and downstream flanking regions for dnd-encoding GIs for E. coli strain UMEA 3176-1 and B7A, slight variations within the highly similar GIs were still observed with transposases found in the variable regions.
The downstream flanking regions of genomic islands for dnd-negative (E. coli CFT073) and dnd-positive (E. coli UMEA 3176-1 and B7A) genomes are also conserved, indicating that the leu tRNA gene site in E. coli is a variable region with other types of GIs found in dnd-negative strains.
Nucleotides not at a filtered diallelic SNPs are from the transcriptome contig (and common to all individuals), while at a filtered diallelic SNPs, the two sequences together represent the called genotype for the individual at that locus (and note that lack of haplotype phasing across such positions does not matter for dnds's calculations).
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