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Inspired by advances in cell surface display technology [22], [23] and peptide fragment library construction [24], we devised a high-throughput scheme that utilizes yeast display and FACS that allows for direct screening of random viral peptide libraries against complex antisera instead of isolated antibodies (Zuo T, Shi XL, Xu WH, Lin Z, and Zhang LQ, unpublished results).
In conclusion, this study substantiates that gross gene abnormalities in PROS1 are relatively common in hereditary PS deficient patients and that MLPA is a useful tool for direct screening of CNVs in PROS1 point mutation-negative individuals.
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A method for direct screen-printing of biological sensors onto clothing has been demonstrated by the University of California San Diego's Laboratory for Nanobioelectronics.
To avoid false positive transformants that may be generated from direct screening for glyphosate tolerance, T-DNA mediated transformants were first screened for kanamycin resistance, a marker carried from the vector pBI121.
We investigated the suitability of surface plasmon resonance (SPR) for providing quantitative binding information from direct screening of a chemical library on protein tyrosine phosphatase 1b (PTP1b).
A fast and simple flow-through optosensor was designed and characterized for the direct screening of four tetracycline (TCC) antibiotics (tetracycline, oxytetracycline, chlortetracycline and doxycycline) in water and bovine milk samples.
Animal cells have been used for the direct screening of hypermutating antibodies [11], [12] and during antibody selection from a retroviral-antibody display library [13].
Dithiane-based photolabile tags have also been used for encoding and direct screening of solution-phase combinatorial libraries.
We sought to expand the known genetic interaction space for important cancer gene orthologs by direct screening for interactions with essential genes.
Using CRISPR/Cas9, plasmid donors with long homology arms (≥1 kb) have been used to insert GFP by coselection for a linked marker or by direct screening for GFP expression (Dickinson et al. 2013; Tzur et al. 2013; Kim et al. 2014).
Finally, we show that our assay can more generally permit simple, direct screening for drugs based on their differential activity against different strains or targets.
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