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Accounting for differential crosslinking efficiencies is a far greater challenge.
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The resulting dual-crosslinked hydrogel swelled anisotropically because of differential crosslink densities between the photopatterned and non-photopatterned regions.
This differential crosslinking provides insight into how TFIID interacts with promoter DNA in the PIC.
All the macroarray membranes were treated following the user manuals, with DNA crosslinked to membranes by baking at 80°C for 2 h, and then were stored at -20°C for differential screenings.
Then you realize you never signed up for Differential Equations.
The numbers show the relative levels for the crosslinking products.
The procedure for TCR crosslinking was described elsewhere.
We used the crosslink Id-Score (Rinner et al., 2008 ) and FDR (Walzthoeni et al., 2012 ) to define three crosslink confidence classes, allowing us to construct a noise model for the crosslinking data.
For crosslinking, yeast cells were treated with 1% formaldehyde for 20 min at room temperature.
For crosslinking reversion, the eluted material was incubated at 65°C for 5 h.
For crosslinking, yeast cells were treated with 1% formaldehyde for 20 min at 25°C.
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