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Blood smears were prepared and stained by the May-Grunewald-Giemsa method for differential counts.
Cells were stained with Quik Diff (Reagena) for differential counts.
Cells were then spun on to slides and stained with Quik-Diff (Reagena) for differential counts.
The normal referenced ranges for differential counts, neutrophils are in the range 15-45 [ 40, 50].
Cell samples were analyzed for differential counts, apoptosis and necrosis using flow cytometry.
For differential counts (lymphocytes, eosinophils, and neutrophils) blood was spread on a clean slide and treated with Leishman's stain before being counted manually with a microscope (100x) [ 19].
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In all patients, besides complete blood count and peripheral blood smear for differential count, circulating CD34+ hematopoietic progenitor cells were enumerated with standard method [5].
The cells were also cytospun onto glass slides for differential counting by using Diff Quick stain (Dade Behring, Newark, DE, USA).
For differential count, the supernatant was discarded after centrifugation (400 g for 10 min at 10°C), and the pellet was resuspended in 300 μL of 3% albumin (Nutricell).
MeDUSA utilises several applications from within the USeq software suite [ 24], and in turn uses the R Bioconductor [ 28] package DESeq [ 29] for differential count analysis.
Blood aliquots were also stained with May-Gründ-Giemsa (InstantProv Kit, NewProv, Brazil), allowing for differential counting of basophils, neutrophils, eosinophils and monocytes by light microscopy.
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