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Stability and reproducibility of the efficiency of the described technique was verified by repetitive analyses of xenografted cells from different mice within the identical passage and for different passages.
Unlike the snare drum, the concert bass drum requires a range of mallet densities for different passages and styles of music.
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The cell lines FK16A and FK18B were established by transfection of primary human foreskin keratinocytes (FK) with the entire HPV16 and HPV18 genome and cultured for different passage number as previously described [ 23].
For each cell line a pool of RNA derived from different passages was obtained, and samples were processed according to the experimental procedures specified by Affymetrix®.
Culture medium was changed every four days for cell growth dynamical analysis, and then growth curves of different passages were drawn.
Not only did this original score, notated for orchestration, have different passages (and that different ending), it was accompanied by a detailed libretto by Prokofiev and the dramatist Sergei Radlov that diverged in important ways from Shakespeare's play.
All of these different passages for communication can be set to various permission levels for different users by the group's administrator.
Reverse transcriptase PCR was performed for individual samples at different passages by designing primers specific to Cytokeratin19 (CK19) and Osteopontin (OPN).
hESC line SA121 was transduced in three separate experiments and at different passages for eGFP expression in undifferentiated cells and EB differentiated cells.
We also screen a large number of prostate cancer cell line as well as primary prostate epithelial cells at different passages for PSCA expression by flow cytometry (Additional file 1: Methods) but were unable to detect any PSCA expression (Additional file 2: Figure S1A).
To validate their NP and AF phenotypes (and hence their tissue origin), we analysed a number of independent primary NP cultures (donors 1 to 5; see Table 1) at different passages for protein and mRNA marker expression (Table 2) and compared them to complementary AF cultures.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com