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Avbelj and Baldwin noticed that solvation strongly affects preferences for different backbone conformations.
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There is evidence for a decrease in flexibility of this loop in the complex, notably at residues Thr173(226) and Ser174(227); in the free enzyme the bundle of structures is divided into two subgroups of different backbone conformation for residues 173 174 creating the high local RMSD values.
To further prove that the presence of the TFS is not necessary for relaxation, plasmids with a different backbone were subjected to TFO−C141S treatment.
"You have to have a different breathing rhythm, a different backbone, a different weight.
The circles show the different backbone linkages of PNA and DNA.
Fig. 2 Energy profile of OMPSi10 with different backbone conformations (empty symbols: 10_opt…10D_opt).
Apparently, the protein structure fluctuates within expected margins, but there is no indication for a systematic long-term trend to escape from the bilayer or to switch to a different backbone fold.
We reason the different response to PRL of reporters is due to the limited proximal promoter regions utilized, the different backbones used for reporter construct, and cells lines used for reporter assay.
This includes self-assembling DNA structures and nucleic acid mimics with different backbones and "bases" for which chemical modification reagents may not be available.
Those molecules have different backbones with saturated (alkane) or conjugated (benzene) structure.
Previously plasmids with different backbones (Dutch and American type backbones) and with and without different tetM determinants (Dutch and American type tetM determinants) have been identified.
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