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Amino acids for diet preparations were purchased from Ajinomoto.
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Procedures for diet preparation and storage were as previously described by Bai and Kim (1997).
Protocols for diet preparation in this study were done as described in the previous study (Hua and Ako 2012).
Draft plans for diet preparation and analysis as well as the study plans encompassing the conduct of feeding trials and the subsequent analysis described above were discussed with 30 stakeholder representatives (some 500 stakeholders invited) in a two-day workshop, and written comments were received from 16 individuals or organisations.
Similarly, Hammond et al. [14] did not present such data but mentioned that all diet preparations were analyzed to confirm that they met the supplier's specifications for certified 5002 rodent diet.
The oils used for the experimental diet preparation were: corn and rapeseed oils (50 50), as the reference diet (ref); hydrogenated coconut oil, as a saturated FA diet (coc); sunflower oil, as an ω6 FA-rich diet (sun); linseed oil, as an ω3 FA-rich diet (lin); and corn, rapeseed and fish oils (42.5 42.5 15), as the fish diet.
The amount of corn in the control diet was adjusted to compensate for the added volume [see 28 for more information on diet preparation and composition].
Oils used for experimental diets preparation were corn and colza oils (50/50) for a reference diet (REF), hydrogenated coconut oil for a saturated fatty acid diet (COC), sunflower oil for an Omega6 fatty acid rich diet (SUN), linseed oil for an Omega3 rich diet (LIN) and corn/colza/enriched fish oils (43/43/14) for the FISH diet.
The experimental diets preparation and storage have been done following Bai and Kim (1997).
Finding the bliss point required the preparation of 61 subtly distinct formulas — 31 for the regular version and 30 for diet.
††Participants screened for diet.
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