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For detection of the effect of growth hormones, the cells were starved in serum-free DMEM/F12 for 16 24 hours prior to the experiment.
For detection of the effect of PKI166 and/or UO126 on phosphorylation of EGFR and ERK1/2, lysates were prepared from cultures of cells that were grown in serum-free medium for 24 h prior to addition of the inhibitors.
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Using simulated data, we demonstrate how the method may be used for early detection of the effect of control measures.
Traditional and new inbreeding coefficients allowed for the detection of the effect of selection on inbreeding [ 27].
This antibody may also have great potential for the detection of the effects of chemopreventive agents in skin cancer.
The ratio can be useful for detection of MNase effect.
Further, Jańczyk et al. (2010) used ion selective electrode for detection of micro-encapsulation effect of pharmaceutical ingredients.
Our sample size was sufficient for detection of moderate effects.
Finally, we demonstrate that immediate processing of tissue samples is crucial for detection of LPS effects.
Thus, we were still faced with lack of power for detection of gene effects.
Furthermore, the use of an internal reference allows for detection of spurious differential effects specific to the HpaII enzyme.
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