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Dey and Chandra [23] proposed two continuous statistical distribution models, gamma and lognormal for desired time gap and time headway of drivers in a steady car-following state on two-lane roads under mixed traffic conditions.
Finally, a simple tuning rule is proposed, which allows to tune the controller for desired time domain properties such as overshoot and rise time, or frequency domain criteria such as stability margin and bandwidth.
Nnd1-dse and the wild type y, w embryos were collected at 18°C for 2 5 hours, aged to desired stages (taking into account the slower developmental rate at this temperature), and then shifted to 29°C for desired time periods before processing them for molecular procedures.
Chambers were assembled and kept in an incubator for desired time points.
Cells cultured in T25 cm flasks were subjected to different drug treatments for desired time intervals and harvested for analysing protein expression.
[ 17] 30 minutes before injection of allantoin; and (IV) the allantoin treated SHRs group (n = 8) treated by intravenous injection of allantoin at various dose for desired time.
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White pre-pupae 0 h APF were collected and grown at appropriate temperatures for desired times before dissection.
The next day, culture medium was replaced with fresh RPMI 1640 containing 10% FBS and 1% penicillin-streptomycin mixture and resveratrol was added for desired times for the measurement of apoptosis and protein expression.
The solution was then kept at room temperature without disturbing for a desired time for aging, after which the UV/vis spectra of the solutions were measured.
Plate cells onto the pre-coated dishes and then incubate at 37°C in 5% CO2 for the desired time appropriate for the signaling response to be analyzed.
After reaction for the desired time (0.5 48 h for metals sorption kinetics at pH 7.5, only 48 h for metals sorption at pH 6.0) the suspension was centrifuged for solid/solution separation.
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